anti- angiogenic activity of vitex agnus castus methanol extract in vivo study

this study aimed to investigate the anti-angiogenic activity of vitex agnus castus methanol extract in vivo. eggs were incubated for three days, small whole made on the fine pinpoint, next day the egg’s sac penetrated and a small frame was made in the shell. the window was resealed and eggs were returned to the incubator until day 10 of chick embryo development, 20 µl of 500mg/ml of the methanol extract transferred to the chick embryo chorioallantoic membrane (cam), and eggs incubated for 72 hours (n = 6); the zone of inhibition calculated as mean of inhibition area in millimetre (mm) ±standard deviation (sd). functional groups of the chemicals component inside the extract has been identified by fourier transform infrared spectroscopy ft-ir and high performance liquid chromatography hplc used to identify the most likely causative agent. the results showed that the zones of inhibition area more than 10 mm. ft-ir showed that some of the identified functional group may relate to flavonoids. casticin has identified in methanol extract. because of the above results the mechanism of anti-angiogenic activity for the methanol extract of the vitex agnus castus may relate to the casticin which has the ability to block the vegf-receptor, thereby inhibiting the angiogenesis process.